Figure 12 of
Boatright, Mol Vis 3:15, 1997.
Figure 12. EMSA of the effects of co-incubation of wild type probe with increasing concentrations of mutant.
Radiolabeled wild type probe was co-incubated with increasing concentrations (0, 0.1, 1, 10, 100, 1000 fold excess) of unlabeled probe that had both Ret-1/PCE-I and the CRX-binding element mutated. The upper bands apparent with both probes in Figure 10 disappear with increasing concentration of competitor. The lower, activator-specific band did not diminish until competitor was at 1000 fold. Co-incubation with the dispersant S-119 had little effect on this response.