Figure 1. Sequences tested in promoter and binding assays.

Mutations of the -70 to -45 region tested in both transient transfection promoter assays and electrophoretic mobility shift assays (EMSA). Sequences are the EMSA probes (sense strands). Mutations are represented by lower case letters.

          Ret-1/    -CBE--
           PCE-I     box
(-70)GCTTGAATTAGACAGGATTAAAGGCT(-45) Wild Type
     GCTTGccggctACAGGATTAAAGGCT      Ret-1/PCE-I Mutant
     GCTTGAATTAGACAGtcggccAGGCT      CRX-binding element (CBE) Mutant
     GCTTGccggctACAGtcggccAGGCT      Ret-1/PCE-I+CBE Mutant