Figure 1 of
Boatright, Mol Vis 3:15, 1997.
Figure 1. Sequences tested in promoter and binding assays.
Mutations of the -70 to -45 region tested in both transient transfection promoter assays and electrophoretic mobility shift assays (EMSA). Sequences are the EMSA probes (sense strands). Mutations are represented by lower case letters.
Ret-1/ -CBE-- PCE-I box (-70)GCTTGAATTAGACAGGATTAAAGGCT(-45) Wild Type GCTTGccggctACAGGATTAAAGGCT Ret-1/PCE-I Mutant GCTTGAATTAGACAGtcggccAGGCT CRX-binding element (CBE) Mutant GCTTGccggctACAGtcggccAGGCT Ret-1/PCE-I+CBE Mutant