Figure 1. Titration of Cell Death-Promoting Activity of Retinal Cell-Conditioned Medium

72 hr conditioned medium was collected from dense cultures of cells death-induced under serumless/defined conditions. Titrations of this conditioned medium were added to fresh R28 cultures for 24 hr. Floating cells were collected, counted in hemacytometer squares, and determined to be greater than 90% dead, by trypan blue analysis. Error bars represent standard deviations.