Figure 5 of
Phillips, Mol Vis 3:12, 1997.
Figure 5. Effects of antisense treatments on glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein levels in E6 and E8 retinal cell cultures
For the representative Western blots, each lane contained 12 µg total protein. The blots were probed with polyclonal antiserum against GAPDH (diluted 1:250) and exposed for 15 min. The area used to quantitate the GAPDH band is indicated by the box. The density scan is of cumulative data from Western blots of three separate experiments probed with anti-GAPDH. Levels of GAPDH in untreated cultures have been normalized and protein levels are expressed as percentages of the control. (C = control not treated with any oligonucleotide; R = treatment with 20 µ M random oligonucleotide; A = treatment with 20 µM antisense oligonucleotide)