Figure 2 of
Schwegler, Mol Vis 3:10, 1997.
Figure 2. Dose-dependence of the bFGF effect on proliferation of human RPE cells in 10% and 1% FCS
The influence of different concentrations of basic fibroblast growth factor (bFGF) at varying time in culture and at high and low fetal calf serum (FCS) concentration on primarily cultured human retinal pigment epithelial (RPE) cells. Cells of the third and fourth passage were grown in 10% FCS (left) and 1% FCS (right), respectively. The vertical axis shows the relative increase of total protein content in a single well of a 96 well microtiter plate. A value of 100% is equivalent to the protein content at each given time in culture without the addition of bFGF. Statistically significant differences in protein content between a treatment and control group are marked with a plus (+) for p<0.05, a double plus (++) for p<0.01, and a triple plus (+++) for p<0.001 (two tailed, unpaired t-test, n=3).