Figure 4. Images of VEGF (green), ZO-1 (red), and nuclei (blue) 1 day after the stencils were removed. A–E: Cells grown under normal conditions had uniform VEGF expression (B) and normal ZO-1 localization (C). F–T: Cells that experienced intercellular junction removal appeared to have a higher expression of VEGF in some locations (arrows) but not consistently across edges of detachment. These cells also had more scattered ZO-1 (H, M, and R). Scale bars: 50 µm.