To access the data, click or select the words “Appendix 3.” Triplex IHC for laminin alpha-5, perlecan and nidogen-1 in 3D cultures prepared with two different batches of epithelial cells. Epithelial cells procured commercially from two different companies—Cell Applications (labeled as Epi2) and Cell Biologics (labeled as Epi 3)—were used to prepare 3D cultures in combination with corneal fibroblasts or myofibroblasts generated from corneal fibroblasts. The fibroblasts and myofibroblasts derived from the same batches of primary cells were used with both lots of epithelial cells for better comparison. The formation of a well assembled epithelial BM, observed via the presence of laminin alpha-5 (green), perlecan (red) and nidogen-1 (yellow) as a thin sheet, only when epithelial cells are cultured in the presence of corneal fibroblasts in the collagen matrix. No evidence for epithelial BM assembly was observed when the epithelial cells are cultured with myofibroblasts. Blue is DAPI stained nuclei. Mag. 200X.