To access the data, click or select the words “Appendix 1.” Representative IHC for laminin alpha-5, perlecan, and nidogen-1 in organotypic cultures prepared with a single cell type. Corneal fibroblasts when cultured alone, showed expression of perlecan and nidogen-1, but very low laminin alpha-5 expression. In corneal myofibroblasts, the expression of laminin alpha-5 and perlecan is less compared to the corneal fibroblasts, with very low to no expression of nidogen-1 in each batch cultured. Blue is DAPI stained nuclei. Mag. 200X.