Figure 8. The corneal application of R9-SOCS3-KIR protected mouse eyes against injury caused by sodium iodate. C57BL/6 mice (10 males and 10 females) were instilled with eye drops containing R9-SOCS3-KIR or its control peptide daily (15 μg in 2 μl in each eye) one day before injecting sodium iodate (25 mg/kg) intraperitoneally. The peptides were administered daily. On day 3, fundoscopy (A) and spectral-domain optical coherence tomography (B) were performed. Hematoxylin- and eosin-stained eyes from the control and treatment eyes (C). Scale bar: 200 μm. The number of infiltrating cells (D) was counted using ImageJ. The thickness of the outer nuclear layer was measured in four different locations (temporal, nasal, superior, and inferior) at a distance of 0.35 mm from the optic nerve head using the segmentation feature of Diver 2.0 (Leica Microsystems) (E). The units are micrometers. Statistical significance was tested using Student’s t-test for unpaired data. ****, p < 0.0001.