Figure 5. ZK002 reduced fibrogenic factor expression in the alkali burned cornea. RT-qPCR showed marked suppression in the mRNA expressions of TGF-β2 (A) and α-SMA (B) in the burned cornea after ZK002 treatment compared with the control group on day 14. Data were expressed as the mean ± SD. Data were analyzed using analysis of variance and Dunnett‘s multiple comparison test. Compared all groups vs. each group. A value of p < 0.05 was considered statistically significant. *p < 0.05, **p < 0.01, ***p < 0.0001. Representative immunohistochemistry of TGF-β2 (C) and α-SMA (D) on day 14 in corneal sections of all groups are shown. Bar=100 µm.