Figure 9. HMGECs were differentiated for 48 h before exposure to latanoprost for 3 h. Lipid extracts were analyzed by ESI-MSMS^ALL (see Methods). Only the TAGs that reached significance are shown. Only 12 of 121 TAGs (9.9%) were significantly altered with latanoprost supplementation. TAG 58:2 (FA 18:1) failed to reach significance in pairwise comparisons. TAGs are labeled by two numbers corresponding to the total number of carbons and the total number of double bonds, respectively. The FA in parentheses represents one of the three FAs of the parent TAG molecule. There were four replicates per condition. HMGEC: human meibomian gland epithelial cell; TAG: triacylglycerol; FA: fatty acid; Gray bar: p ≤ 0.05; Black bar: p ≤ 0.01; Dashed bar: p ≤ 0.001.