Figure 15. HMGECs were differentiated for 48 h before exposure to combined latanoprost-BAK for 3 h. Lipid extracts were analyzed by ESI-MSMS^ALL (see Methods). Only the TAGs that reached significance are shown. Thirty-six of the 121 TAGs (29.8%) were significantly different among latanoprost alone, BAK alone, or combined latanoprost-BAK. However, only three TAGs (TAG 50:2 [FA 18:1], TAG 52:0 [FA 16:0], and TAG 52:1 [FA 16:1]) showed significant differences between the combined latanoprost-BAK and each component in isolation. TAGs are labeled by two numbers corresponding to the total number of carbons and the total number of double bonds, respectively. The FA in parentheses represents one of the three fatty acids of the parent TAG molecule. There were four replicates per condition. BAK: benzalkonium chloride; CE: cholesteryl esters; Gray bar: p ≤ 0.05; Black bar: p ≤ 0.01; Dashed bar: p ≤ 0.001.