Figure 14. HMGECs were differentiated for 48 h before exposure to combined latanoprost-BAK for 3 h. Lipid extracts were analyzed by ESI-MSMS^ALL (see Methods). The inset depicts only the CEs that reached significance. Five of the 50 CEs (10.0%) were significantly different between latanoprost alone, BAK alone, or combined latanoprost-BAK. CE 28:2 failed to reach significance in pairwise comparisons. No CEs showed significant differences between the combined latanoprost-BAK and each component in isolation. CEs are labeled by carbon number and double-bond count, respectively. When a third number is present, it denotes an oxCE with the corresponding number of oxygenations. There were four replicates per condition. BAK: benzalkonium chloride; CE: cholesteryl ester; oxCE: oxidized cholesteryl ester; Gray bar: p ≤ 0.05; Black bar: p ≤ 0.01.