**Figure 5. **Blocking ERK1/2 signaling prevented Wnt/β-catenin signal transduction in LEC under moderate oxidative stress. **A**: Inactivation of ERK1/2 signaling inhibited p-GSK-3β^{Ser9} and β-catenin. **B**: Intracellular domain (ICD) of LRP6, five PPPS/TP motifs within ICD are highlighted, and Ser 1490 is marked in red. **C**: Inactivation of ERK1/2 signaling inhibited LRP6 phosphorylation at Ser 1490 (*p<0.05 and **p<0.01). Statistical analysis
was conducted with the paired Student *t* test using the integrated density of the protein band. The data are shown as mean ± standard error of the mean (SEM) with
n = 3.