Figure 5. Blocking ERK1/2 signaling prevented Wnt/β-catenin signal transduction in LEC under moderate oxidative stress. A: Inactivation of ERK1/2 signaling inhibited p-GSK-3β^Ser9 and β-catenin. B: Intracellular domain (ICD) of LRP6, five PPPS/TP motifs within ICD are highlighted, and Ser 1490 is marked in red. C: Inactivation of ERK1/2 signaling inhibited LRP6 phosphorylation at Ser 1490 (*p<0.05 and **p<0.01). Statistical analysis was conducted with the paired Student t test using the integrated density of the protein band. The data are shown as mean ± standard error of the mean (SEM) with n = 3.