Figure 4. Blocking ERK1/2 signaling prevented moderate H₂O₂-induced EMT in LECs. A: Western blotting of epithelial-mesenchymal transition (EMT) biomarkers, including E-cadherin, vimentin, α-SMA, and Snail. B: Immunofluorescent image of vimentin. Statistical analysis was performed with the paired Student t test using the integrated density of the protein band (*p<0.05 and **p<0.01). The data are shown as mean ± standard error of the mean (SEM) with n = 3.