**Figure 2. **Exposing LECs to 50 μM of H_{2}O_{2} for 48 h activated Wnt/β-catenin signaling and promoted EMT. **A**: Western blotting of E-cadherin, vimentin, α-SMA, and Snail. **B**: Western blotting of Wnt 10a, p-LRP6^{Ser1490}, p-GSK-3β^{ser9}, and β-catenin. **C**: Immunofluorescent images of β-catenin. Statistical analysis was performed with the paired Student *t* test (*p<0.05 and **p<0.01). The data are shown as mean ± standard error of the mean (SEM) with n = 3.