Figure 6. The Ig0 domain of basigin-1 does not stimulate the expression of IL-6. Interleukin-6 (IL-6) present in the cell culture medium of (A) RAW 264.7 or (B) HEK293–7 cells treated with D-PBS, C-6XHis, Ig0–6XHis, Ig0N-6XHis, or Ig0L1–6XHis recombinant proteins (5 μM) or mouse neural retina protein lysate (NR, 500 μg/ml) for 24 h at 37 °C was measured via quantitative enzyme-linked immunosorbent assay (ELISA) and plotted as the mean concentration (pg/ml) of triplicate runs. The error bars represent the standard deviation of the mean. A Kruskal–Wallis test was performed. The p values are indicated (α level = 5%).