Figure 5. RAW 264.7 cells express basigin-2 and LPS-induced IL-6. A: Immunocytochemical analyses of RAW 264.7 using an antibody specific for basigin gene products [10] and Alexa 488-conjugated goat-anti-rabbit secondary antibody. Basigin-2 expression is represented by green fluorescence, and nuclear DNA is represented in purple. The magnification bar represents 100 μm. B: Interleukin-6 (IL-6) present within the cell culture medium of RAW 264.7 cells treated with D-PBS or lipopolysaccharide (LPS: 1 μg/ml) for 24 h at 37 °C was measured via quantitative enzyme-linked immunosorbent assay (ELISA) and plotted as the mean concentration (pg/ml) of triplicate runs. The error bars represent the standard deviation of the mean. An unpaired t test was performed. The p values are indicated (α level = 5%).