Figure 5. Validation and comparison of mRNA and circular RNA (circRNA) expression levels between quantitative real-time PCR (qRT-PCR) and RNA-seq. A, C: The expression levels of five mRNAs (CALD1, RHOA, AES, CTSD, and LAPTM5) by RNA sequencing analysis (A) and qRT-PCR (C). B, D: The expression levels of three circRNAs (circRNA_2189, circRNA_3654, and circRNA_3192) by RNA sequencing analysis (B) and qRT-PCR (D).