Figure 3. The antiviral effect of SPS on HSV-1 after preincubation. HSV-1g was preincubated with SPS for 1 h at 37°C, and the mixture was then used to infect HCE-T cells for 1 h at 37°C, followed by washing and culturing in a base medium for another 24 h. (A) HSV-1g replication was detected using fluorescence microscopy. (B) The average fluorescence intensity was measured using ImageJ software. The average intensity of the virus control was assigned a value of 100. Data are presented as the means ± standard deviations (n = 3). **p < .01 compared with the control group.