Figure 3. Exchange protein activated by cAMP 1 (Epac1) can overcome hydrogen peroxide (H2O2)-mediated activation of NLR family pyrin domain containing 3 (NLRP3) pathway proteins. Western blot for retinal endothelial
cells (RECs) grown in normal glucose (NG) and treated with H2O2 or an Epac1 agonist (Panel A). Panels B–D are Western blot results for NLRP3 (B), ASC1 (C), and cleaved caspase 1 (D). Panel
E shows the ELISA results for IL-1β in the same treatments. n = 6. Data are mean ± SEM *p < 0.05 versus NG, # p < 0.05 versus
NG + H2O2.