Figure 3. Chronic unpredictable mild stress (CUMS) induced neuronal cell loss in the ganglion cell layer (GCL) of the retina. A: The representative area of the selected central and peripheral retina in the whole mount. B: The representative neuronal nucleus (NeuN) staining. C: Quantification of NeuN-positive cells in the central and peripheral retina in whole mounts at the end of 5 weeks of CUMS exposure. The amount of neural cells in the retina was lower in the CUMS mice than it was in the normal mice in the central and peripheral retina. Data are presented as mean ± standard error of the mean (SEM), n = 6–6. *p<0.05, **p<0.01. Scale bar = 50 um.