Figure 4. HCECs were pretreated with BT or ZnPP for 2 h, followed by 2 h incubation with 10 nM LXA4 and A. fumigatus stimulation. qRT-PCR and ELISA results showed that the LXA4-induced downregulation of A,B: TNF-α, C, D: IL-1β, and E,F: IL-6 mRNA and protein levels at 8 and 20 h post-stimulation in HCECs was partially blocked by BT or ZnPP pretreatment. The increment of HO-1 protein level elicited by LXA4 was suppressed at 8 h post-infection by G,H: BT pretreatment. All data are presented as mean ± SD and evaluated by an unpaired, two-tailed Student t test (ns: no significance).