Figure 2. Effects of LXA4 on the expression of pro-inflammatory mediators in A. fumigatus–stimulated HCECs. Cells were pretreated with 1 and 10 nM LXA4 for 2 h, followed by 8 h or 20 h A. fumigatus stimulation. qRT-PCR quantification of mRNA levels of A: TNF-α , C: IL-1β, and E: IL-6. ELISA analysis of protein levels of B: TNF-α, D: IL-1β, and F: IL-6. All data are presented as mean ± SD and evaluated by an unpaired, two-tailed Student t test (ns: no significance).