Appendix 4 of Farkas, Mol Vis 2022; 28:394-411.
To access the data, click or select the words “Appendix 4.” Analysis of EMT-associated genes in RNA-Seq data. A. DESeq normalized counts for known EMT-associated genes in DYS, CW1, and A2 iPSC-RPE. All of the EMT-associated genes, excluding ZEB1, are expressed to some degree in all three sample types. B. Nearly all of the EMT-associated genes are upregulated in the SLOS-derived (CWI and A2) are significantly upregulated relative to the DYS control line. The exceptions are SNAI1, SNAI2, and ZEB1 in the CWI:DYS comparison. Additionally, all, but one (CDH1), are significantly upregulated in the more severe SLOS sample (A2) compared to the CWI sample, which has a milder phenotype. INF=expression is observed in only one sample of the comparison.