To access the data, click or select the words “Appendix 1.” Immunohistochemical analysis of occludin (green; anti-occludin, mAb OC3-F10) and cytochemical analysis of F-Actin (red; Alexa Fluor 568- conjugated phalloidin) of DYS, CWI, and A2 RPE cells. DYS RPE cells formed continuous monolayers constituted by polygonal cells with sharp occludin-positive cell borders. The F-actin cytoskeleton of DYS RPE cells appeared mostly lateral and cortical. In CWI RPE cells, occludin-positive cell borders often appeared irregular (yellow asterisks), with intermittent phalloidin-labeled F-actin stress fibers (white arrows). A2 RPE cells were highly heterogeneous, consisting of some occludin-negative, or discontinuous monolayer regions with aberrant, pleomorphic cell shapes, as well as regions of regularly arranged polygonal cells with occludin-positive cell contacts (yellow asterisks). A2 RPE monolayers exhibited varied F-actin stress fiber formation (white arrows). Scale bar: 20 μm.