Figure 3. MnSOD knockdown decreases retinal thickness as measured with OCT, and SFN improves retinal thickness in Nrf2 KO mice. A–B: spectral domain-optical coherence tomography (SD-OCT) was used to measure and compare retinal thickness in the eyes of WT and Nrf2 KO mice before any treatments. Error bars represent SEM (standard error of the mean). Representative SD-OCT images of WT (C) and Nrf2 KO (D) eyes before subretinal injections. Arrows in (D) denote disruptions to the RPE layer observed in a subset of Nrf2 KO mice before the AAV1-RzSOD2 injection. Graphs of SD-OCT measurements taken 1 month (E) and 4 months (F) after AAV1-RzSOD2 delivery (i.e., 3 months after SFN treatment was initiated). Statistical significance (p<0.05) was determined with an unpaired Student t test or one-way ANOVA using GraphPad Prism.