Figure 5. Relative expression levels of PRPF31 and CNOT3 in TB209 family members. The PRPF31 (A) and CNOT3(B) expression levels in blood lymphocytes (measured in triplicate) were normalized to the housekeeping gene GAPDH. The error bars refer to the standard deviation of the mean for each individual. ave, average; +, wt; M, mutant; UA, unaffected; AF, affected, N.S., not significant. *p < 0.05.