Figure 9. Construction of the CRYAA knock down HLECs. A: CRYAA mRNA expression was significantly down-regulated in HLECs transfected with CRYAA-sh1, CRYAA-sh2, CRYAA-sh3 and CRYAA-sh4. B: Expression of CRYAA protein was determined with western blot assays and the CRYAA protein expression was significantly up-regulated in CRYAA-sh1 transduced with wild type CRYAA plasmid and CRYAA mutant E156K plasmid.