Figure 9. Changes in choroidal vascularization after the laser impacts and nestin siRNA injection. A: No apparent changes in vascularization was observed in the control animals during the experimental period (upper panels). On the seventh day after the laser treatment, the angiographic fluorescent intensity increased considerably. On the 14th day, the fluorescence was still intense, with the appearance of dense microvascular networks at the site of the laser impact (upward thin arrow). The nestin siRNA injection reduced the fluorescence intensity, even if some micro-vessel islands were still present on the 14th day (rightward thick arrow). These results are confirmed by the measurement value of the vascular density calculated using the Fiji/Image J analysis software on the B: second day, C: seventh day, and D: 14th day after the laser impacts.