Figure 2. Retinal and choroidal nestin (green) co-immunolabeling with glial fibrillary acidic protein (GFAP, red), glutamine synthetase (GS, red), or von Willebrand factor (vWF, red) in the control retina on the seventh day post laser treatment. A–C: GFAP (red) and nestin (green). A: GFAP is present in astrocytes (upward thin arrow) and around some blood vessels (thick arrow) in the GCL and OPL. B: Nestin was detected in cells in the same retinal layers where the GFAP was detected. C: These results were confirmed by co-immunolabeling with nestin/GFAP. D–F: GS (red) and nestin (green). D: GS immunoreactivity can be observed in Müller cells (upward thin arrow). E: Nestin was detected in cells (probably in quiescent astrocytes) in the GCL (upward thin arrow) and rarely in some cells with radial processes (downward thin arrow). Some blood vessels appeared also partially labeled (leftward thick arrow). F: Nestin/GS co-immunolabeling is never observed in functional mature Müller cells (leftward thick arrow) and blood vessels (rightward thick arrow). G–I: vWF (red) and nestin (green). G: vWF immunolabeling was found in blood vessels in the retina (downward thin arrow and upward “plug” arrow) and choroid (downward thick arrow). H: Nestin immunolabeling was observed in the GCL (downward thin arrow and upward “plug” arrow), in the OPL (thin arrow), and at the choroid level (downward thick arrow). I: vWF/nestin co-immunolabeling was found in the GCL (upward “plug” arrow), in the OPL (downward thin arrow), and in the choroid (downward thick arrow). Scale bars correspond to 50 μm. GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, OPL: outer plexiform layer, ONL: outer nuclear layer, Ch: Choroid.