To access the data, click or select the words “Appendix 1.” Immunofluorescent labeling with DAPI (blue) and nestin (green) is shown. No morphological changes are observed at 150μm from laser impacts and in control rats with no laser impacts, as observed with DAPI staining (A, C). In these conditions, nestin immunostaining is seen in processes of astrocytes and in probable endfeet of Muller cells located mostly in the GCL (horizontal left orientated thin arrow; D). They are also observed in structures similar to blood vessels located in retina and choroid (horizontal large arrows; B D). At the impact, DAPI labeling (E) demonstrated that retinal morphology is greatly disrupted in the outer retina with cells of the ONL entering the choroid (large vertical thick arrow). Nestin immunostaining is observed in glial cells in GCL (thin arrow), in retinal radial processes (horizontal large arrow) and in processes located in choroid (large star; F). All pictures were taken from different retinal sections. GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, OPL: outer plexiform layer, ONL: outer nuclear layer, Ch: choroid. Scale bars correspond to 50μm.