Figure 3. Overexpression of miR-598-3p causes repressed proliferation ability of retinoblastoma (RB) cells. After Y79 and HXO-RB44 cells
were transfected with miR-598-3p mimic or miR-598-3p inhibitor, (A) reverse transcription quantitative polymerase chain reaction (RT-qPCR) was applied to inspect the transfection effect, (B) cell counting kit-8 (CCK-8) assay, and (C) 5-ethynyl-2’-deoxyuridine (EdU) staining (50X) to measure cell proliferation ability, (D) flow cytometry to examine the apoptotic rate, and (E) western blotting to detect the expression of apoptosis-related proteins Bax and Bcl-2 in Y79 and HXO-RB44 cells. *p<0.05,
**p<0.01.
