Figure 2. MALAT1 targets and negatively regulates miR-598–3p. The lowly expressed miR-383-5p, miR-503-5p, and miR-598-3p in Y79 cells
and HXO-RB44 cells were screened with (A) reverse transcription quantitative polymerase chain reaction (RT-qPCR). The (B) online software TargetScan predicted the binding site of MALAT1 to miR-598-3p, and the mutation site was designed. The interaction
between MALAT1 and miR-598-3p was verified with the luciferase (C) reporter assay. The regulatory effect of MALAT1 on miR-598-3p in Y79 cells and HXO-RB44 cells was examined with (D) RT-qPCR. *p<0.05, **p<0.01.