Figure 1. Overexpression of MALAT1 promotes retinoblastoma (RB) cell proliferation. After RB cells were transfected with pcDNA3.1-MALAT1
or sh-MALAT1. A: Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was applied to detect the differential expression
of MALAT1 in ARPE-19 cells and RB cell lines Y79, SO-RB50, HXO-RB44, WERI-RB-1, and SO-RB70. B: Then, the transfection efficiencies of pcDNA3.1-MALAT1 and sh-MALAT1 in Y79 and HXO-RB44 cells were evaluated with (B) RT-qPCR. The (C) cell counting kit-8 (CCK-8) assay and (D) 5-ethynyl-2’-deoxyuridine (EdU) staining (50X) were employed to assess cell proliferation ability, (E) flow cytometry for inspection of the cell apoptotic rate, and (F) western blotting for measurement of the expression of apoptosis-related proteins Bax and Bcl-2 in Y79 and HXO-RB44 cells.