Appendix 2 of Lin, Mol Vis 2022; 28:269-279.

Appendix 2. MALAT1 acts through the PI3K/AKT pathway

To access the data, click or select the words “Appendix 2.” Notes: Y79 cells were transfected with pcDNA3.1-MALAT1, sh-MALAT1, pcDNA3.1-MALAT1 + LY294002, or sh-MALAT1 + 740 Y-P. CCK-8 assay (A) and EdU staining (B, × 50) were employed to measure the proliferation ability of Y79 cells. Flow cytometry was adopted to assess the apoptotic rate of Y79 cells (C). Western blotting was applied to detect the expression of apoptosis-related proteins Bax and Bcl-2 in Y79 cells (D). Western blotting was used to examine the expression of PI3K/AKT pathway-related proteins in Y79 cells (E). **p<0.01, *p<0.05.