Figure 9. Aggregate formation by internalized FITC-labeled αAP1, αBP4, and scrambled peptides in human lens epithelial cells (HLEB3) in culture. The cells were permeabilized and then treated with Proteostat Aggresome dye. In the cells, individual peptides alone showed green fluorescence, and red fluorescence by aggregated peptides reactive to Proteostat Aggresome dye, along with cellular nuclei (stained blue with Hoechst stain). The merged image shows the overlay of the green and red fluorescence of cells. Each experiment was performed in triplicate, and 100 cells with either red or green fluorescence or both were manually counted with a hemocytometer using a Leica fluorescent microscope.