Figure 4. Effect of peptides on the chaperone activity of αA-crystallin using insulin as the target protein. Effect of the five selected peptides—(A) αAP1, (B) αAP2, (C) αBP3, (D) αBP4, and (E) αBP5—on the chaperone activity of αA-crystallin using insulin as the target protein. In the assay, 100 µM of individual peptides with recombinant αA-crystallin (100 µg each) with insulin (200 µg) as a target protein plus dithiothreitol (DTT) were incubated at 37 °C for 60 min. Absorbance at 360 nm was recoded every 15 min.