Figure 3. Determination of anti-chaperone activity of selected peptides. Antichaperone activity determination of the five selected peptides—(A) αAP1, (B) αAP2, (C) αBP3, (D) αBP4, and (E) αBP5—using insulin as the target protein. The effect of various concentrations of the peptides (5, 25, 50, and 100 µM) in aggregation of insulin (200 µg) in the presence of dithiothreitol (DTT) at 37 °C for 60 min was determined. Absorbance at 360 nm was recoded every 15 min.