Figure 4. TNFAIP3 siRNA increased inflammatory mediators. Retinal endothelial cells (RECs) grown in normal glucose (NG) or high glucose (HG). Some cells in HG were treated with scrambled siRNA or TNFAIP3 siRNA. Western blotting was performed for TNFα (A) and phosphorylated NF-kB to total NF-kB (B), and phosphorylated to total IkB (C). *p<0.05 versus NG, #p<0.05 versus HG. Data are mean ± standard error of the mean (SEM) n = 5.