Figure 3. TNFAIP3 siRNA decreased ZO-1 and occludin levels. Retinal endothelial cells (RECs) grown in normal glucose (NG) or high glucose (HG). Some cells in HG were treated with scrambled siRNA or TNFAIP3 siRNA. Western blotting was performed for TNFAIP3 (A), ZO-1 (B), and occludin (C). Panels D and E show immunostaining for tight junction protein 1 (D) and ZO-1 (E). *p<0.05 versus NG, #p<0.05 versus HG. Data are mean ± standard error of the mean (SEM) n = 5.