Figure 6. Outflow facility and Mmp gene expression were significantly enhanced in protein-treated PlatKO mouse eyes. A: Outflow facility in protein-treated PlatKO mouse eyes. Outflow facility (mean ± SD µl/min/mmHg) was significantly increased in eyes treated with tPA (n = 12) and NE-tPA (n = 13) compared to those treated with BSA (n = 19; ** p<0.01, ANOVA with Tukey-Kramer post hoc analysis). Group means of gene expression changes in Mmp-9 (C) and Mmp-13 (D) were significantly different in PlatKO BSA eyes (n = 8), tPA eyes (n = 12), and NE-tPA eyes (n = 8; ANOVA, Mmp-9, p<0.0001, Mmp-13, p<0.0001). Group means of gene expression changes in Mmp-2 (B) were not significantly different across treatment groups (ANOVA, Mmp-2, p>0.05). Asterisks indicate differences in Tukey-Kramer post hoc analysis, * p<0.05, **p<0.01, *** p<0.001, ****p<0.0001.