Figure 5. Outflow facility and Mmp gene expression were significantly enhanced in protein-treated C57BL/6 mouse eyes. A: Outflow facility in TA- and protein-treated C57BL/6 mouse eyes. Outflow facility (mean ± SD µl/min/mmHg) was significantly increased in eyes treated with TA+tPA (n = 15) and TA+NE-tPA (n = 10) compared to those treated with TA+BSA (n = 18; **** p<0.0001, ANOVA with Tukey-Kramer post hoc analysis). The outflow facility of naïve (not treated with either TA or protein) C57BL/6J animals is included for comparison. The outflow facility in these eyes was similar to the outflow facility in tPA- and NE-tPA-treated eyes. Gene expression changes in Mmp-2 (B), Mmp-9 (C), and Mmp-13 (D) were normalized (mean ± SD) to values from naïve eyes. Group means were significantly different in TA+BSA eyes (n = 4), TA+tPA eyes (n = 8), and TA+NE-tPA eyes (n = 8; ANOVA, Mmp-2, p<0.0001, Mmp-9, p<0.0001, Mmp-13, p<0.01). Asterisks indicate differences in Tukey-Kramer post hoc analysis, * p<0.05, **p<0.01, ***p<0.001 ****p<0.0001.