Figure 4. Outflow facility was significantly enhanced in adenovirus-treated C57BL/6 mouse eyes. A: Outflow facility in TA- and adenovirus-treated C57BL/6J mouse eyes. Outflow facility (mean ± SD µl/min/mmHg) was significantly increased in eyes treated with TA+AdPLAT (n = 11) and TA+AdPLATNE (n = 8) compared to those treated with TA+AdNull (n = 11; **** p<0.0001, ANOVA with Tukey-Kramer post hoc analysis). The outflow facility of naïve (not treated with either TA or adenovirus) C57BL/6 animals is included for comparison. The outflow facility in these eyes was similar to the outflow facility in AdPLAT- and AdPLATNE-treated eyes. Gene expression changes in Mmp-2 (B), Mmp-9 (C), and Mmp-13 (D) were normalized (mean ± SD) to values from naïve eyes. Expression was significantly different between TA+AdNull eyes (n = 16) and both TA+AdPLAT eyes (n = 16) and TA+AdPLATNE eyes (n = 8). Naïve eyes (n = 20) also had lower expression than the TA+AdPLAT- and TA+AdPLATNE-treated eyes. Asterisks indicate differences on Tukey-Kramer post hoc analysis, ** p<0.01, ***p<0.001, ****p<0.0001. mRNA was below detectable limits for Mmp-2, Mmp-9, and Mmp-13 in the TA+AdNull group.