Figure 2. Quantification of tPA enzymatic activity. tPA enzymatic activity in supernatants of non-transfected HMVECs (n = 3) and HMVECs transfected with either pShuttle-CMV-PLAT (n = 3) or pShuttle-CMV-PLATNE (n = 4). tPA enzymatic activity in supernatant from pShuttle-CMV-PLAT transfected cells was significantly higher than the activity in supernatant from pShuttle-CMV-PLATNE transfected and non-transfected cells (p<0.01, ANOVA). Asterisks indicate differences on Tukey-Kramer post hoc analysis, * p<0.05, ** p<0.01.