Figure 4. AQP5 deficiency induced inflammation in the lacrimal gland. A: Hematoxylin and eosin (H&E) staining showed morphology changes. CD4⁺: Immunofluorescence staining of CD4⁺ demonstrated CD4⁺ T cells (red: CD4⁺, blue: 4’6-diamidino-2-phenylindole [DAPI]). Ly6g: Immunofluorescence staining of Ly6g showed neutrophils (red: Ly6g, blue: DAPI). F4/80: Immunofluorescence staining of F4/80 showed macrophages (red: F4/80, blue: DAPI). B: ImageJ immunofluorescence profile analysis showed the CD4⁺, Ly6g, and F4/80 positive scores (n = 6 samples). C: Real-time PCR results exhibited chemokine and proinflammatory factors (n = 3 samples). *p<0.05, **p<0.001, two-way analysis of variance (ANOVA). Scale bars: (A) 40 μm. *p<0.05, **p<0.01, ***p<0.0001.