Figure 3. AQP5^−/− induced abnormal lipid metabolism in the lacrimal gland. A: Oil Red O (ORO) staining (n = 6 samples). B: ORO staining intensity analyzed by ImageJ software (n = 6 samples). C,D,E: Real-time PCR analyzed the expression of PPARα, CPT1α, and CPT2 (n = 3 samples). *p<0.05, **p<0.001, two-way analysis of variance (ANOVA). Scale bars: (A) 40 μm. * p<0.05, ** p<0.01, *** p<0.0001.