Figure 1. AQP5 deficiency induced dry eye–like characteristics. A: Immunofluorescence staining showed the expression of AQP5 in the lacrimal gland cell location (green: AQP5, blue: 4’6-diamidino-2-phenylindole [DAPI]). B: Expression of AQP5 and GAPDH were examined by western blotting. C: Bodyweight (n = 10 samples). D: Lacrimal gland weight and E: appearance (n = 8 samples). F: Tear secretion of AQP5^+/+ mice and AQP5^−/− mice. G: Sodium fluorescein staining of AQP5^+/+ mice and AQP5^−/− mice. Scale bars: (A) 40 μm. * p<0.05, ** p<0.01, *** p<0.0001.