Figure 2. Tunnel assay indicates an increase in Tunnel-positive cells (white arrowheads) in the Western diet (WD) fed Ossabaw mini pigs (B) compared to the Standard diet (SD) fed pigs (A). Panels C and D are negative controls where Tdt enzyme and Anti-Digoxigenin Conjugate were respectively excluded during the staining procedure. Arrowheads indicate the apoptotic cells.