Figure 5. DEX exposure induces alteration in DNA methylation in hRPE cells. A: Changes in the genomic DNA methylation level of human RPE (hRPE) cells exposed to 1 mg/ml and 2 mg/ml dexamethasone (DEX) after 48 h. The transcription level of the DNA methyltransferases (DNMTs) (B–D) was determined with quantitative real-time PCR (qRT-PCR) after 48 h of DEX exposure. E: Changes in the genomic DNA hydroxymethylation level of the hRPE cells exposed to 1 mg/ml and 2 mg/ml of DEX after 48 h. The transcription levels of ten-eleven translocations (TETs) (F–H) were determined with qRT-PCR after 48 h of DEX exposure. *p<0.05, **p<0.005 with the Student two-tailed t test compared to the control group. n=3.